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2018年4月11日 星期三
Reed-Muench Method
1.
計算距離值
[(感染力高於50%之佰分比)–50% ] / [(感染力高於50%之佰分比) – (感染力低於50%之佰分比)]
2.
計算TCID50
取大於50% CPE之稀釋倍數之log值加上距離值
3.
Example (第一排取10 μl病毒液稀釋到90 μl的培養基中)
10-1
|
10-2
|
10-3
|
10-4
|
10-5
|
10-6
|
10-7
|
10-8
|
|
S1
|
+
|
+
|
-
|
-
|
-
|
-
|
-
|
-
|
S2
|
+
|
+
|
+
|
-
|
-
|
-
|
-
|
-
|
S3
|
+
|
+
|
+
|
-
|
-
|
-
|
-
|
-
|
S4
|
+
|
+
|
+
|
-
|
-
|
-
|
-
|
-
|
S5
|
+
|
+
|
+
|
+
|
-
|
-
|
-
|
-
|
Control
|
-
|
-
|
-
|
-
|
-
|
-
|
-
|
-
|
Ratio
|
100%
|
100%
|
80%
|
20%
|
0%
|
0%
|
0%
|
0%
|
距離值 (80% - 50%) / (80% - 20%) = 0.5
TCID50 = 10 (3+0.5) * 100= 105.5
= 3.16 x 105 / ml
標籤: Edu, Immunology, Statistics
1. 開啟PS
2. [編輯] --> [偏好設定] --> [camera raw]
3. 找到最下方[效能] --> [使用圖形處理器] --> 取消打勾
4. [確定] --> 重開PS即可