無風的日子

1. 開啟MEGA4
2. Alignment --> Query Databanks (0r Do blast search)
3. 在Web browser視窗 --> Keyword --> Go , Display --> fasta , Send to --> Text , add to Alignment
4. 在Alignment Explorer視窗 --> Alignment --> align by ClustalW, File --> save session
5. 在MEGA4視窗 --> Open Data --> XX.meg , phylogeny --> Construct Phylogeny --> 選擇模式


Mega5
1. Align icon --> do blast search --> Add to Alignment
2. Alignment --> Align by ClustalW
2.a  manual edit alignment data --> del the gap region
3. Data --> export alignment -->  Mega format
4. Exit Alnexpoloer


5. Open a file --> XXX.meg
6. Models icon --> finds best DNA/protein models
7. Phylogeny icon --> construct/test ML tree
8. select  substitution model from (6) and  test --> Bootstrap method